Chapter 1, operating instructions, contains basic operating instructions for using the dsq ii mass spectrometer detector. Merge width is used only for the lcqtsqquantumicis and dsqpolarisqgcq. Even if a few atoms in a sample of chlorine, for example, captured an electron instead of losing one, the negative ions formed wouldnt get all the way through the ordinary mass spectrometer. Precursor to modern mass spectrometer developed mass spectrograph developed discovery of the electron figure 1 a timeline showing important advances in mass spectrometry over the last century. The contents of the solvent spray, the gas flow rate, the amount of applied voltage, the spray angle and the ion uptake angle, as well as the various distances in aligning the spray, sample and mass spectrometer are all variables which can be studied to achieve an optimal mass spectrum for a particular type of sample. Gc, which acts as a separation technique, and ms, which acts as a. Before installing the easynlc vi control software, do the following. We have 4 thermo scientific trace 10 manuals available for free pdf download. Please read carefully the operating manuals before starting the bluebox and isa. Samples can be introduced via gc column or direct insertion probe dip. Identification and characterization of proteins by mass. Agilent 6545xt advancebio lcq tof data sheet parameter measure specification ms sensitivity, positive signaltonoise for 1 pg injection of reserpine, while maintaining maximum mass resolution 500. Mass spectrometry is a method for finding out the mass of particles contained in a sample and, thereby, for identifying what those particles are.
Inside a mass spectrometer, a sample of test material is converted into a highspeed stream of electrically charged particles. The trace dsq is a powerful quadrupole mass spectrometer ms detector, with features benefiting those in the chemical science industryanalysts, technicians, and chemists. View and download thermo scientific dsq ii hardware manual online. In adj param, choose temperatures, to check if the source temperature is at the set temperature. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures. Thermo scientific content manual and documents official. Finnigan tracedsq gcms analyze detect measure c ontrol fullscan sensitivity for the demanding environmental analysis proven performance for dif.
I n early d ays, spectrometers were simply prism having graduation marking wavelengths. Page preface this guide contains detailed instructions for operating, maintaining, and troubleshooting the dsq ii mass spectrometer. This chapter contains basic operating instructions for using the dsq ii mass spectrometer. The waters micromass qtof micro mass spectrometer the micromass qtof micro is a high resolution oatof mass spectrometer that enables automated exact mass measurements in an easytouse benchtop instrument.
Siltite gc connections are designed to minimize installation time and provide ongoing, robust connections throughout the life of the gc column. Complex mixtures of individual compounds can be injected into the gc, either manually or through the use of an optional autosampler, and then separated for. The lcms system consists of the easynlc instrument, a thermo scientific mass spectrometer, and a data system computer. Dsq ii, designed for analysis of small amounts of sample in dif. The instrument also features a quadrupole mass filter and collision cell for msms analyses. The instrument also features a quadrupole mass filter. The gc or lc separates the components in a mixture, and the components are introduced, one by one, into simple schematic of a mass spectrometer interfaced to a gc. The epa method 8270 productivity solution thermo pn 120291kit, was used for this. The thermo dsq ii dual stage quadruple, circa 2006, and dsq, circa 2002, based on the trace msplus, are single quad mss that interface to. A mass spectrometer or mass spectrograph consists of three essential parts. A trace dsq system includes the trace dsq ms detector, the trace gc, and the xcalibur software ver. Product specifications dsq ii single quadrupole gcms.
You should replace the inlet valve seal periodically. Manual tune for mass spectrometer haverford college. Choose thermo scientific isq series quadrupole gcms systems for time proven. Used thermo dsq ii mass spectrometer for sale dotmed. Take care of your source maintenance without venting the system with the.
Spectrometer is a broad term often used to describe instruments that measure a continuous variable of a phenomenon where the spectral components are somehow mixed. In the top window, and tune ms menu, choose manual tune. Media backgrounder high level information for journalists thermo fisher scientific in mass spectrometry much of science is devoted to answering two questions. Column connections in the gc have traditionally suffered from unreliability, leaks, excessive dead volumes and lack of intertness, leading to poor chromatography results and instrument downtime. Thermo scientific gas chromatography mass spectrometry gcms systems enable identification of volatile and semivolatile compounds at trace levels and. Manuals and user guides for thermo scientific trace 10. Install compatible versions of the thermo foundation platform and the xcalibur data system or equivalent thermo scientific data system. Thermo scientific dsq ii hardware manual pdf download. Gc, which acts as a separation technique, and ms, which acts as a detection technique. Inlet valve seal thermo scientific dsq ii hardware manual.
The appendix contains dsq ii drawings and part numbers for replaceable parts and consumables. The results are typically presented as a mass spectrum, a plot of intensity as a function of the mass tocharge ratio. Mass spectrometry ms is an analytical technique that measures the mass tocharge ratio of ions. Mass spectrometry body, chemical, energy, gas, parts, basic. Certificate of analysis mass spectrometry and separations. Please help us improve the quality of our documentation by completing and returning this survey. Dsq ii gcms systems obtain improved detectivity without sacrificing dynamic range or. The innovations of the dsq ii, including curved prefilter, durabrite source, and dynamax xr detection system, offer. Supplies and services for the thermo dsqtrace mass. The innovations of the thermo scientific dsq ii, including curved prefilter, durabrite xl source, and dynamax xr detection system, offer fundamental improvements in your analytical results. Equipment, installation category ii, pollution degree 2. The inlet valve knob allows you to tighten the seal against the ir tool to form a vacuumtight seal or you can loosen it to allow the ir tool to be inserted and removed. Mass spectrometers tandem mass spectrometry msms mass spectrometers are commonly combined with separation devices such as gas chromatographs gc and liquid chromatographs lc. Dsq ii hardware manual, 1202990002, revision c free gift for your returned readers survey.
View online or download thermo scientific trace 10 instruction manual, installation manual. Xcalibur qualitative analysis user guide planet orbitrap. Onaxis optics dsq ii offaxis optics continuing over 30 years of gcms excellence, thermo electron corporation introduces the dsq ii gcms series, instruments that will change the way you think about gcms analysis. Mass spectrometry is the technology of choice used to answer these questions in a. Mass spectrometry ms is an analytical technique that measures the masstocharge ratio of ions. Wilmington police department crime laboratory quality management system technical procedure forensic drug analysis standard operating procedure. Block diagram of a mass spectrometer ion source detector.
Thermo scientific trace dsq gcms mass spectrometry. Ltq orbitrap velos is a hybrid mass spectrometer incorporating the. For budgetmindful lab owners seeking a reliable replacement for older gcms systems or standalone gcs, the isq 7000 gcms system is a robust solution that delivers operational simplicity and proven dependability. The system has both electron impact ei and chemical. Gas chromatography mass spectrometry gcms thermo fisher. Score the column with a scribing tool approximately 50 mm 2 in. Autosamplers ai 10 and as 10 manuals english ai 3000 and as 3000 manuals english triplus classic manuals english triplus 100 ls manuals english triplus rsh manuals english triplus 300 hs manuals english gas chromatographs trace 0 series manuals english trace 0 series installation notes english focus gc manuals english trace gc ultra manuals english mass. About your system gas chromatographymass spectrometry gcms represents a combination of two powerful analytical techniques. Highlighted are instrument developments blue, applications of mass spectrometry gray and general scientific achievements green. No part of this manual may be reproduced in any form. All mass spectrometers that you will come across if you are doing a course for 16 18 year olds work with positive ions.
In order to aid you in the selection of replacement parts for your instrument, the following part number cross reference chart can be used. Resolve the mass separation of isotopes for the metal ions from any of a number of single isotope or multiisotope. Isq preinstallation requirements guide pn 1r1205550001 isq hardware manual pn 1r1205550002 isq user guide pn 1r1205550003 isq spare parts guide pn 1r1205550004 isq and tsq 8000 direct probe system user guide pn. Your dsq ii instrument provides you with the highest caliber gas chromatography mass spectrometry gcms instrumentation available on todays market. The trace dsq is a powerful quadrupole mass spectrometer ms detector. Thermo scientific dsq ii single quadrupole mass spectrometer. The combination of noise reduction and increased source and detector performance provide unrivaled analytical performance across. The results are typically presented as a mass spectrum, a plot of intensity as a function of the masstocharge ratio.
Refurbished thermo dsq ii single quadrupole gcms large capacity vacuum pump bundle complete with focus gc including a single splitsplitless injector, rv3 vacuum pump, and windows xp pc with xcalibur 2. Equipped with the new innovative durabrite source and dynamax xr detection system, the dsq ii series is able to detect. Thermo electron corporation dsq ii hardware manual xi how to use this guide refer to the chapter containing the instructions you are interested in. This note describes a technique for the analysis of these two compounds using automated headspacespme sampling in conjunction with the thermo scienti. A simple description of how a mass spectrometer works. If you are interested in understanding the purpose of this electric field, see the mass spectrometer page. Hardware manual, instruction manual, installation manual. Supplies and services for the thermo dsqtrace mass spectrometer. The thermo dsq ii dual stage quadruple, circa 2006, and dsq, circa 2002, based on the trace msplus, are single quad mss that interface to trace gc or focus gc family. Refurbished thermo dsq ii single quadrupole gcms iet.
Forensic drug analysis standard operating procedure. A typical application of mass spectrometry is the identification of small amounts of materials found at a crime scene. Single quadrupole gcms thermo fisher scientific us. On their way, they hit atoms or molecules in the sample, and knock one or more electrons off to give positive ions. Thermo scientific trace 10 manuals manuals and user guides for thermo scientific trace 10. Offaxis optics in the dsq ii provide a quantitative reduction in neutral noise versus a straightline quadrupole system. About your system thermo fisher scientific systems provide the highest caliber gas. In a nutshell, the electric field, in conjunction with the magnetic field, selects ions of a particular velocity that will pass into the semicircular region. Trace dsq hardware manual, pn 1201560002, revision b. Product specifications dsq ii mass spectrometer durabrite source electron energy adjustable between 0 and ev and emission current up to 850 a independently controlled heating from 125 300 c for stable operation and chromatographic integrity noncoated inert source material for ei, pci, and nci dualstage quadrupole mass. Ltq orbitrap velos hardware manual brown university. A mass map mass fingerprint is a set of fragment masses obtained by digestion of a protein by a specific usually enzymatic reaction followed by direct mass spectrometric analysis of the resulting protein fragmentsthe analysis is usually performed by malditoftof mass spectrometry and the obtained mass spectrum can be.
The number of hits may also be reduced by taking an accurately measured isotope pattern of the ion into consideration. Thermo electron corporation dsq ii hardware manual ix preface this manual contains detailed instructions for operating, maintaining, and troubleshooting your instrument. Choose thermo scientific isq series quadrupole gcms systems for timeproven. It is also designed to reduce the contact of target compounds with active sites in the column, which leads to a reduction in the residence time of analytes and increases the speed of analysis. Resolve the mass separation of isotopes for the metal ions from any of a number of single isotope or multiisotope salts such as sodium chloride or potassium chloride. C he m g ui d e an s we r s the mass spectrometer 1. Block diagram of a mass spectrometer ion source mass analyzer detector high vacuum sample in computer 400 600 800 1200 1400 1600 1800mz 0 20 40 60 80 100 relative abundance mass analyzer activation or reaction tandem mass spectrometry or msms. Complex mixtures of individual compounds can be injected into the. Gc, which acts as a separation technique and ms, which acts as a detection technique. Xcalibur, trace dsq, focus dsq, and polarisq are trademarks andor product. Isq series mass spectrometers hardware manual revision e.
This guide contains detailed instructions for operating, maintaining, and troubleshooting the dsq ii mass spectrometer. Gas chromatography mass spectrometry gc ms represents a combination of two powerful analytical techniques. Product specifications dsq ii mass spectrometer durabrite source electron energy adjustable between 0 and ev and emission current up to 850 a independently controlled heating from 125 300 c for stable operation and chromatographic integrity noncoated inert source material for ei, pci, and nci dualstage quadrupole mass filter. Providing versatility and exceptional gc and gcms performance, its an ideal choice for larger production and qaqc laboratories and research organization. Thermo scientific trace 10 hardware manual 624 pages gas chromatographs. Chapter 2, maintenance instructions, contains maintenance. The energy spectrum of particles of known mass can also be measured by determining the time of flight between two detectors and hence, the velocity in a timeofflight. Please enter the lot number of your product and you will be guided to the respective certificate of analysis coa.
A mass spectrometer is an analytical instrument that is used to identify the amount and type of chemicals present in a sample by measuring the masstocharge ratio and abundance of gasphase ions timeofflight spectrometer. These will be considered separately and then followed with a series of example applications. In the inactive spectrum view, drag the cursor from mz 198. It really doesnt matter how advanced the technology in the mass spectrometer is if the gc cannot deliver the sample reliably and robustly. About your system gas chromatography mass spectrometry gcms represents a combination of two powerful analytical techniques. Boost productivity, accelerate response times, and lower your total cost of ownership with the thermo scientific trace 10 gas chromatograph. Chapter 2 getting started, provides gcms instructions using xcalibur. Scientific instrument services carries a wide selection of parts for all models of thermofinnigan mass spectrometers. In 1900s the first spectrometer was developed by joseph john thomson at cavendish laboratory and his student francis william aston, built a mass spectrometer in 1919. Help us improve the quality of our manuals by circling one number for each statement. Mass spectrometry body, chemical, energy, gas, parts.
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